AB Bioscience LLC
"The Home of KlenTaq1"
 Formerly AB Peptides since 1992
Woman Owned Small Business
Authorized Vendor to US Gov

GF Klentaq1 Cat# 1003

(A Glycerol-Free version of Klentaq1 DNA Polymerase)

Amount: 100 µl (sufficient for 2000 25 µl reactions up to 1 kb)

Shipping conditions: Ice pack

Storage conditions: Enzyme at 2-8°C, buffer -20°C

*Note: Do not freeze enzyme.

Shelf life: Not determined. (Suggesting the enzyme to be used soon after delivery.)



Klentaq1 is a 5'-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability. 10x buffer composition is: 500 mM Tris-Cl pH 9.2, 160 mM ammonium sulfate, 0.5% Brij 58, and 35 mM magnesium chloride. We also offer (upon request) 10x buffer at pH 7.9 for better fidelity.

TYPICAL PCR PROTOCOL for a 25 ml reaction:



Final Concentration

10x Klentaq1 Reaction buffer

2.5 ml


dNTP mix (10 mM)

0.5 ml

200 mM each

Left Primer


0.2 mM

Right Primer


0.2 mM

DNA template


0.1-100 ng




Klentaq1 Polymerase**

0.05 ml


de-ionized distilled H2O

Adjust final volume to 25 ml


DNA amount depends mostly on genome size and target gene copy number.

**To determine specific optimal enzyme concentration, we strongly recommend an enzyme titration test for each target. Targets larger than 1 kb may require more enzyme or may benefit from the use of an LA (Long Accurate) version of the polymerase.




1. Denaturing:                      94° for 2 minutes for 1 cycle

2. Denaturing:                     94° for 20-30 seconds

3. Annealing:                        50°-68° depending on the specific Tm primers for 40-60 seconds

4. Extension:                        68° for 2 min / 1kb target

5. Repeat steps 2-4 for 25-40 cycles






Barnes, W.M. (1994) PCR amplification of up to 35 kb DNA with high fidelity and high yield from bacteriophage templates, PNAS 91, 2216-2220.


U.S. Patent No. 5,436,149