AB Bioscience LLC
"The Home of KlenTaq1"
 Formerly AB Peptides since 1992
Woman Owned Small Business
Authorized Vendor to US Gov


Klentaq1 Cat#1001


 

Amount: 100 µl (sufficient for 2000 25 µl reactions up to 1 kb)

Shipping conditions: Ambient temperature but ice packs are included

Storage conditions: Enzyme and buffer -20°C

Shelf life: At least 3 years from date of receipt under proper storage conditions.

 


TYPICAL PCR PROTOCOL for a 25 ml reaction:

Reagent

Volume

Final Concentration

10x Klentaq1 Reaction buffer

2.5 ml

1x

dNTP mix (10 mM)

0.5 ml

200 mM each

Left Primer

variable

0.2 mM

Right Primer

variable

0.2 mM

DNA template

variable

0.1-100 ng

PCR enhancer (optional)

6.25-12.5 µl

 

Klentaq1 Polymerase**

0.05 ml

2.5 units

de-ionized distilled H2O

Adjust final volume to 25 ml

-

DNA amount depends mostly on genome size and target gene copy number.

**To determine specific optimal enzyme concentration, we strongly recommend an enzyme titration test for each target. Targets larger than 1 kb may require more enzyme.

 

 

CYCLING CONDITIONS

1. Denaturing:                      94° for 2 minutes for 1 cycle

2. Denaturing:                      94° for 20-30 seconds

3. Annealing:                        50°-68° depending on the specific Tm primers for 40-60 seconds

4. Extension:                        68° for 2 min / 1kb target

5. Repeat steps 2-4 for 25-40 cycles

 

 

 

 

REFERENCES:

Barnes, W.M. (1994) PCR amplification of up to 35 kb DNA with high fidelity and high yield from bacteriophage templates, PNAS 91, 2216-2220.

 

U.S. Patent No. 5,436,149


Please order at:

order@abpeps.com


Tel :  314 968-4944 

Fax : 314 968-8988


AB BIOSCIENCE, LLC. 

8224 Manchester Road Ste 101, St. Louis, MO 63144

Domestic or International S/H charges will apply.